.WAF

Pueblo High School

University of Arizona

Biotechnology Course

Summer School

Top Ten Reasons to Take Biotechnology Class this Summer

Goal: After completionof this class, students will understand and appreciate the opportunities available for a career in Biotechnology. From the Arizona Daily Star's New Econony page "Biotech benefits: From an industry standpoint, biotechnology has much to offer. It's clean and non-polluting. And it pays well, with jobs starting in Tucson at $40,000 to $50,000 a year. "I really believe the defining technology of the coming decade will be in biotechnology," says Eugene Sander, dean of the University of Arizona College of Agriculture. "That's going to be as important as the silicon chip was over the last three decades." http://www.azstarnet.com/neweconomy/biotech1.html

 

 Syllabus

  1. Skill Building: Spectrophotometry, Gel Electrophoresis, PCR, Microbiology, and Pipetting
  2. Review metric system e. g. microliters
  3. DNA and Biotechnology Timeline
  4. Size of Genome
  5. Extraction and Quantitation of DNA
  6. DNA Replication
  7. Polymerase Chain Reaction
  8. Genetically Modified Foods
  9. Cost of Doing Biotechnology $$$$
  10. Readings from Genes we share with Yeast, Flies, Worms and Mice (www.hhmi.org)

Attendance Policy: Students may be dropped if they miss 1.5 days during the session. Three tardies equal 0.5 days.

Student Arrival

***

Before 7:10 AM

on time (congratulations)

From 7:11 to 8 AM

Tardy

From 8:01 to 10 AM

0.5 days missed

After 10:00 AM

1 day missed

Tentative Schedule

May 24 (Monday)
May 25 (Tuesday)
May 26 (Wednesday)
May 27 (Thursday)
May 28 (Friday)

 
  1. Initial Assessment
  2. Classrooom Procedures
  3. Principles of Biotechnology
  4. DNA: molecule of the century, Part 1
  5. safety contract
  6. Electrophoresis 1 (Background and Bromothymol blue)
  1. Test
  2. metrics
  3. Pipetting- large volume
  4. Pipetting- small volume
  5. DNA: molecule of the century, Part 2
 
  1. Test
  2. Electrophoresis 2 (pour gels, separation of dyes)
  3. DNA structure (model)
  4. video (Cracking the Genetic Code)
  1. Test
  2. Mitochondrial DNA introduction
  3. DNA Goes to the Races
  4. DNA isolation (student)
  5. DNA Fingerprint Lab I
May 31 (Monday)
June 1 (Tuesday)
June 2 (Wednesday)
June 3 (Thursday)
June 4 (Friday)
Memorial Day
  1. Test
  2. Lecture-Restriction enzymes
  3. DNA Restriction Digest Lab
  4. DNA Scissors
  5. Fingerprint Homework
  1. Test and article: Palo Verde
  2. Lecture-Restriction enzymes and restriction mapping-1
  3. Transformation Lab (green fluorescent protein-GFP) Day 1
  1. Test
  2. GFP Transformation Lab Day 2
  3. Looper experiment- Day 1
  4. Central Dogma
  5. How to produce GM crops
  1. Test
  2. Fingerprint Homework Due
  3. Looper experiment- Day 2
  4. DNA replication (PCR preview)
  5. Paper PCR
  6. GFP Transformation Lab Day 3
  7. GFP extraction: Lessons 1 and 2
June 7 (Monday)
June 8(Tuesday)
June 9 (Wednesday)
June 10 (Thursday)
June 11 (Friday)
  1. Test
  2. Homework: Cost of Biotechnology
  3. DNA message conversion activity
  4. Transcription: Control of gene expression (operon-arabinose)
  5. GFP extraction: Lesson 3
  6. Looper experiment- Day 3
  1. Test
  2. Bt cotton plant PCR
  3. GFP extraction: Lessons 4 and 5
  1. Test
  2. New Techniques 1: Microarrays, proteomics, SARS))
  3. video
  4. article
  1. Final Assessment
  2. Size of genome (ALU elements and "junk" DNA)
 

 Tentative Grading

Points

A 540-600 points

Daily Lab Work 20 points 12x20= 240 points

B 480-539 points

Daily Tests 20 points 12x20= 240 points

C 420-479 points

Homework 60 points 2x60= 120 points

D 360-419 points

Total Points= 600 points

F < 359 points

 

 

We are done!