Pueblo High School

Goal: After completionof this class, students will understand and appreciate the opportunities available for a career in Biotechnology. From the Arizona Daily Star's New Econony page "Biotech benefits: From an industry standpoint, biotechnology has much to offer. It's clean and non-polluting. And it pays well, with jobs starting in Tucson at $40,000 to $50,000 a year. "I really believe the defining technology of the coming decade will be in biotechnology," says Eugene Sander, dean of the University of Arizona College of Agriculture. "That's going to be as important as the silicon chip was over the last three decades." http://www.azstarnet.com/neweconomy/biotech1.html

 Syllabus

1. Skill Building: Spectrophotometry, Gel Electrophoresis, PCR, Microbiology, and Pipetting
2. Review metric system e. g. microliters
3. DNA and Biotechnology Timeline
4. Size of Genome
5. Extraction and Quantitation of DNA
6. DNA Replication
7. Polymerase Chain Reaction
8. Genetically Modified Foods
9. Cost of Doing Biotechnology $$$$
10. Readings from Genes we share with Yeast, Flies, Worms and Mice (www.hhmi.org)

Attendance Policy: Students may be dropped if they miss 1.5 days during the session. Three tardies equal 0.5 days.

Tentative Schedule

May 26 (Monday)	May 27 (Tuesday)	May 28 (Wednesday)	May 29 (Thursday)	May 30 (Friday)
		Initial Assessment Classrooom Procedures DNA: molecule of the century, Part 1 safety contract Electrophoresis 1 (Background and Bromothymol blue) video (Cracking the Genetic Code)	Test DNA Timeline metrics Pipetting Electrophoresis 2 (pour gels, separation of dyes) article: Palo Verde Fingerprinting	Test DNA structure (model) DNA isolation (kiwi) Restriction enzymes 1 DNA Fingerprint Lab DNA Fingerprint Homework
June 2 (Monday)	June 3 (Tuesday)	June 5 (Thursday)	June 6 (Friday)
Test Review of Homework Introduction to genetically modified (GM) crops (SMITH) Looper experiment- Day 1 Central Dogma Spectrophotometry 1 Bitter Harvest-video	Test Speaker- David Thorpe, MD PhD Introduction of Microbiological techniques (SMITH) Spectrophotometry 2- Standard Curve Looper experiment- Day 2 DNA quantitation 1 Restriction enzymes 2	Test How to produce GM crops Looper experiment- Day 3 Transformation Lab (green fluorescent protein-GFP) Day 1 DNA replication (PCR preview)	Test GFP Transformation Lab Day 2 DNA quantitation 2 Bt cotton PCR Day 1 Looper experiment- Day 4 >Transcription: Control of gene expression (operon-arabinose) (Kuemmerle)	Test Looper experiment- Day 5 Homework: Looper Lab report Bt cotton PCR Day 2 GFP Transformation Lab Day 3 GFP extraction: Lessons 1 and 2
June 9 (Monday)	June 10 (Tuesday)	June 11 (Wednesday)	June 12 (Thursday)	Administrative Team does PCR
Test PCR simulation GFP extraction: Lesson 3 Restriction enzymes Lab Part1 GM crops- issues (SMITH) video article	Test Restriction enzymes Lab Part2 GFP extraction: Lessons 4 and 5 video article	Test New Techniques 1: Microarrays, proteomics, SARS) (Kuemmerle) ALU PCR 1 video article	Final Assessment Size of genome (ALU elements and "junk" DNA) ALU PCR 2

Assessment

• Name and label the three parts of a nucleotide

 

We are done!