.WAF

Pueblo High School

University of Arizona

Biotechnology Course

Read about it in the ARIZONA DAILY STAR "Students dabble in DNA" by David Wichner from 7/3/03

(at Duke's Place)

Summer School

Goal: After completionof this class, students will understand and appreciate the opportunities available for a career in Biotechnology. From the Arizona Daily Star's New Econony page "Biotech benefits: From an industry standpoint, biotechnology has much to offer. It's clean and non-polluting. And it pays well, with jobs starting in Tucson at $40,000 to $50,000 a year. "I really believe the defining technology of the coming decade will be in biotechnology," says Eugene Sander, dean of the University of Arizona College of Agriculture. "That's going to be as important as the silicon chip was over the last three decades." http://www.azstarnet.com/neweconomy/biotech1.html

 Syllabus

  1. Skill Building: Spectrophotometry, Gel Electrophoresis, PCR, Microbiology, and Pipetting
  2. Review metric system e. g. microliters
  3. DNA and Biotechnology Timeline
  4. Size of Genome
  5. Extraction and Quantitation of DNA
  6. DNA Replication
  7. Polymerase Chain Reaction
  8. Genetically Modified Foods
  9. Cost of Doing Biotechnology $$$$
  10. Readings from Genes we share with Yeast, Flies, Worms and Mice (www.hhmi.org)

Attendance Policy: Students may be dropped if they miss 1.5 days during the session. Three tardies equal 0.5 days.

Student Arrival

***

Before 7:10 AM

on time (congratulations)

From 7:11 to 8 AM

Tardy

From 8:01 to 10 AM

0.5 days missed

After 10:00 AM

1 day missed

Tentative Schedule

June 4 (Wednesday)
May 26 (Monday)
May 27 (Tuesday)
May 28 (Wednesday)
May 29 (Thursday)
May 30 (Friday)

 
  1. Initial Assessment
  2. Classrooom Procedures
  3. DNA: molecule of the century, Part 1
  4. safety contract
  5. Electrophoresis 1 (Background and Bromothymol blue)
  6. video (Cracking the Genetic Code)
 
  1. Test
  2. DNA Timeline
  3. metrics
  4. Pipetting
  5. Electrophoresis 2 (pour gels, separation of dyes)
  6. article: Palo Verde Fingerprinting
  1. Test
  2. DNA structure (model)
  3. DNA isolation (kiwi)
  4. Restriction enzymes 1
  5. DNA Fingerprint Lab
  6. DNA Fingerprint Homework
June 2 (Monday)
June 3 (Tuesday)
June 5 (Thursday)
June 6 (Friday)
  1. Test
  2. Review of Homework
  3. Introduction to genetically modified (GM) crops (SMITH)
  4. Looper experiment- Day 1
  5. Central Dogma
  6. Spectrophotometry 1
  7. Bitter Harvest-video
  1. Test
  2. Speaker- David Thorpe, MD PhD
  3. Introduction of Microbiological techniques (SMITH)
  4. Spectrophotometry 2- Standard Curve
  5. Looper experiment- Day 2
  6. DNA quantitation 1
  7. Restriction enzymes 2
  1. Test
  2. How to produce GM crops
  3. Looper experiment- Day 3
  4. Transformation Lab (green fluorescent protein-GFP) Day 1
  5. DNA replication (PCR preview)
  1. Test
  2. GFP Transformation Lab Day 2
  3. DNA quantitation 2
  4. Bt cotton PCR Day 1
  5. Looper experiment- Day 4
  6. >Transcription: Control of gene expression (operon-arabinose) (Kuemmerle)
  1. Test
  2. Looper experiment- Day 5
  3. Homework: Looper Lab report
  4. Bt cotton PCR Day 2
  5. GFP Transformation Lab Day 3
  6. GFP extraction: Lessons 1 and 2
June 9 (Monday)
June 10 (Tuesday)
June 11 (Wednesday)
June 12 (Thursday)
Administrative Team does PCR
  1. Test
  2. PCR simulation
  3. GFP extraction: Lesson 3
  4. Restriction enzymes Lab Part1
  5. GM crops- issues (SMITH)
  6. video
  7. article
  1. Test
  2. Restriction enzymes Lab Part2
  3. GFP extraction: Lessons 4 and 5
  4. video
  5. article
  1. Test
  2. New Techniques 1: Microarrays, proteomics, SARS) (Kuemmerle)
  3. ALU PCR 1
  4. video
  5. article
  1. Final Assessment
  2. Size of genome (ALU elements and "junk" DNA)
  3. ALU PCR 2

 Tentative Grading

Points

A 540-600 points

Daily Lab Work 20 points 12x20= 240 points

B 480-539 points

Daily Tests 20 points 12x20= 240 points

C 420-479 points

Homework 60 points 2x60= 120 points

D 360-419 points

Total Points= 600 points

F < 359 points

 

Assessment

 

We are done!